Contact persons

Priscilla Sanabria, Ph.D., Director

The facility offers microscope-based systems that allow the assessment of cellular responses, such as calcium signaling with fast temporal resolution.  The facility offers 1) Confocal imaging services; 2) Brightfield, darkfield, phase contrast, Nomarski and epi-fluorescence imaging; 3) High spatial and temporal resolution imaging, 4) Collection of serial sections (Deconvolution capabilities) and 5) Morphometric analysis.

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Services

  • DIC, brightfield and fluorescence imaging (Material analysis service) In live and fixed specimens.
  • Intracellular Calcium recording in live cells (Material processing service)
  • Preparation of specimens (Material processing service) Service includes: vibratome tissue preparation, fixation, permeabilization and antibody optimization.

Instrumentation/software

  • Hamamatsu ORCA-ER C4747-95-12ERG (Digital camera) Leica VT1000S Vibratome (Vibratome) New Brunswick Scientific CO2 incubator (CO2 incubator) Olympus 1X70 inverted microscope
  • (Inverted microscope) Olympus BH2 Fluorescence Microscope (Fluorescence microscope) Olympus DP71 microscope digital camera (Microscope digital camera) Olympus Fluoview FV1000 confocal
  • microscope (Confocal microscope) Perkin Elmer Wallac 1420 Victor 2 (Scintillation counter) Photometrics CoolSnap HQ CCD Camera (Charge-coupled device camera) Photon Technology
  • International spectrofluorometer (Fluorometer) Sutter Lambda 10-2 optical filter (Optical filter) Sutter Lambda DG-4 (Optical filter)